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Magnesium Sulfate Has Potential Scavenging Effects on Cyclophosphamide-Induced Ovarian Damage in A Rat Model
Received: 06 Feb 2019 | Received in revised form: 26 Feb 2019
Accepted: 28 Feb 2019 | Available online: 19 Mar 2019
J Clin Obstet Gynecol. 2019;29(1):24-30
DOI: 10.5336/jcog.2019-65377
Article Language: EN
Article Language: EN
Copyright Ⓒ 2024 by Türkiye Klinikleri. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
ABSTRACT
Objective: This study proposed to investigate whether magnesium sulfate (MgSO4) could reduce the ovarian damage induced by cyclophosphamide (Cyc). Material and Methods: Thirty female rats were used for this study: Control group (n=10)- Only laparotomy; Cyc group n=10)-75 mg/kg Cyc intraperitoneally; and Cyc+MgSO4 group (n=10)-75 mg/kg Cyc on day 0 and 200 mg/kg MgSO4 on days 1-7 (both intraperitoneally). The extent of histopathological damage and the number of ovarian follicles were determined. The levels of anti-Mullerian hormone (AMH) were measured in blood samples. Results: Statistically significant differences in the AMH values were observed in the control group versus Cyc group and Cyc group versus Cyc+MgSO4 group (p<0.05). The levels of AMH were the least in the Cyc group. The total tissue damage in the Cyc group was significantly higher than that in the control, as well as in the Cyc+MgSO4 group (p<0.05). The follicle counts were the least in the Cyc group. Conclusion: Cyc caused ovarian damage and reduced the ovarian reserves. The ovarian reserves in the MgSO4-treated group were better than those in the other groups, and there was least ovarian damage in the MgSO4-treated group.
Objective: This study proposed to investigate whether magnesium sulfate (MgSO4) could reduce the ovarian damage induced by cyclophosphamide (Cyc). Material and Methods: Thirty female rats were used for this study: Control group (n=10)- Only laparotomy; Cyc group n=10)-75 mg/kg Cyc intraperitoneally; and Cyc+MgSO4 group (n=10)-75 mg/kg Cyc on day 0 and 200 mg/kg MgSO4 on days 1-7 (both intraperitoneally). The extent of histopathological damage and the number of ovarian follicles were determined. The levels of anti-Mullerian hormone (AMH) were measured in blood samples. Results: Statistically significant differences in the AMH values were observed in the control group versus Cyc group and Cyc group versus Cyc+MgSO4 group (p<0.05). The levels of AMH were the least in the Cyc group. The total tissue damage in the Cyc group was significantly higher than that in the control, as well as in the Cyc+MgSO4 group (p<0.05). The follicle counts were the least in the Cyc group. Conclusion: Cyc caused ovarian damage and reduced the ovarian reserves. The ovarian reserves in the MgSO4-treated group were better than those in the other groups, and there was least ovarian damage in the MgSO4-treated group.
REFERENCES:
- Tomao F, Spinelli GP, Panici PB, Frati L, Tomao S. Ovarian function, reproduction and strategies for fertility preservation after breast cancer. Crit Rev Oncol Hematol. 2010;76(1):1-12. [Crossref] [PubMed]
- Chapman RM. Effect of cytotoxic therapy on sexuality and gonadal function. Semin Oncol. 1982;9(1):84-94. [PubMed]
- Oktem O, Oktay K. Quantitative assessment of the impact of chemotherapy on ovarian follicle reserve and stromal function. Cancer. 2007;110(10):2222-9. [Crossref] [PubMed]
- Carlsson IB, Scott JE, Visser JA, Ritvos O, Themmen AP, Hovatta O. Anti-Mullerian hormone inhibits initiation of growth of human primordial ovarian follicles in vitro. Hum Reprod. 2006;21(9):2223-7. [Crossref] [PubMed]
- Pigny P, Merlen E, Robert Y, Cortet-Rudelli C, Decanter C, Jonard S, et al. Elevated serum level of anti-mullerian hormone in patients with polycystic ovary syndrome: re- lationship to the ovarian follicle excess and to the follicular arrest. J Clin Endocrinol Metab. 2003;88(12):5957-62. [Crossref] [PubMed]
- Coskun B, Dilbaz B, Karadag B, Coskun B, Tohma YA, Dur R, et al. The role of anti-Mul- lerian hormone in predicting the response to clomiphene citrate in unexplained infertility. Taiwan J Obstet Gynecol. 2018;57(5):713-7. [Crossref] [PubMed]
- Piltonen T, Morin-Papunen L, Koivunen R, Perheentupa A, Ruokonen A, Tapanainen JS. Serum anti-Müllerian hormone levels re- main high until late reproductive age and de- crease during metformin therapy in women with polycystic ovary syndrome. Hum Re- prod. 2005;20(7):1820-6. [Crossref] [PubMed]
- Kao MC, Jan WC, Tsai PS, Wang TY, Huang CJ. Magnesium sulfat emitigates lung injury induced by bilateral lower limb ischemia-reper- fusion in rats. J Surg Res. 2011;171:97-106. [Crossref] [PubMed]
- Akan M, Ozbilgin S, Boztas N, Celik A, Ozkardesler S, Ergur BU, et al. Effect of mag- nesium sulfate on renal ischemia-reperfusion injury in streptozotocin-induced diabetic rats. Eur Rev Med Pharmacol Sci. 2016;20(8): 1642-55. [PubMed]
- Itoh K, Maki T, Shindo A, Egawa N, Liang AC, Itoh N, et al. Magnesium sulfate protects oligo- dendrocyte lineage cells in a rat cell-culture model of hypoxic-ischemic injury. Neurosci Res. 2016;106:66-9. [Crossref] [PubMed] [PMC]
- Lemos CN, Reis FM, Pena GN, Silveira LC, Camargos AF. Assessment of fertility pro- tection and ovarian reserve with GnRH an- tagonist in rats undergoing chemotherapy with cyclophosphamide. Reprod Biol En- docrinol. 2010;8:51. [Crossref] [PubMed] [PMC]
- Saleh DO, Mansour DF. Ovario-protective effects of genistein against cyclophos- phamide toxicity in rats: role of anti-müller- ian hormone and oestradiol. Eur J Pharmacol. 2016;789:163-71. [Crossref] [PubMed]
- Gürgen SG, Erdoğan D, Elmas C, Kaplanoğlu GT, Ozer C. Chemoprotective effect of ascor- bicacid, ?-tocopherol, and selenium on cy- clophosphamide-induced toxicity in the rat ovarium. Nutrition. 2013;29(5):777-84. [Cross - ref] [PubMed]
- Tsuyoshi H, Orisaka M, Fukuda S, Hattori K, Tsang BK, Yoshida Y. Protective effect of dienogest on chemotherapy-induced reduced fertility in female rats. Steroids. 2015;93:1-7. [Crossref] [PubMed]
- Yener NA, Sinanoglu O, Ilter E, Celik A, Sez- gin G, Midi A, et al. Effects of spirulina on cy- clophosphamide-induced ovarian toxicity in rats: biochemical and histomorphometric evaluation of the ovary. Biochem Res Int. 2013;2013:764262. [Crossref] [PubMed] [PMC]
- Visser JA, de Jong FH, Laven JS, Themmen AP. Anti-Müllerian hormone: a new marker for ovarian function. Reproduction. 2006;131(1): 1-9. [Crossref] [PubMed]
- Yeh J, Kim B, Liang YJ, Peresie J. Müllerian inhibiting substance as a novel biomarker of cisplatin-induced ovarian damage. Biochem Biophys Res Commun. 2006;348(2):337-44. [Crossref] [PubMed]
- van Rooij IA, Broekmans FJ, te Velde ER, Fauser BC, Bancsi LF, de Jong FH, et al. Serum anti-Müllerian hormone levels: a novel measure of ovarian reserve. Hum Reprod. 2002;17(12):3065-71. [Crossref] [PubMed]
- Dayangan Sayan C, Tulmac OB, Karaca G, Ozkan ZS, Yalcin S, Devrim T, et al. Could erythropoietin reduce the ovarian damage of cisplatin in female rats? Gynecol Endocrinol. 2018;34(4):309-13. [Crossref] [PubMed]
- Atli M, Engin-Ustun Y, Tokmak A, Caydere M, Hucumenoglu S, Topcuoglu C. Dose depend- ent effect of resveratrol in preventing cisplatin- induced ovarian damage in rats: an experimental study. Reprod Biol. 2017;17(3): 274-80. [Crossref] [PubMed]
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