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The Beneficial Effects of Vitamin D3 Against Trichloroethylene Toxicity in Rat Ovaries
Received: 22 Mar 2020 | Received in revised form: 02 Jun 2020
Accepted: 12 Jun 2020 | Available online: 11 Aug 2020Helin BAĞCI a, Rauf MELEKOĞLUb, Ebru ÇELİK KAVAKc, Tuncay KULOĞLUd, Mehmet ŞİMŞEKe
aKarabük Training and Research Hospital, Department of Obstetrics and Gynecology, Karabük, TURKEY
bİnönü University Faculty of Medicine, Department of Obstetrics and Gynecology, Malatya, TURKEY
cFırat University Faculty of Medicine, Department of Obstetrics and Gynecology, Elazığ, TURKEY
dFırat University Faculty of Medicine, Department of Histology and Embryology, Elazığ, TURKEY
eAdıyaman University, Department of Obstetrics and Gynecology, Adıyaman, TURKEY
J Clin Obstet Gynecol. 2020;30(2):65-72
DOI: 10.5336/jcog.2020-75002
Article Language: EN
Copyright Ⓒ 2024 by Türkiye Klinikleri. This is an open access article under the CC BY-NC-ND license (
http://creativecommons.org/licenses/by-nc-nd/4.0/)
ABSTRACT
Objective: Vitamin D3 is an active metabolite of vitamin D and has been reported to have anti-oxidative effects due to inhibition of lipid peroxidation in some tissues both in vitro and in vivo studies. The current study was carried out to assess the protective effects of vitamin D3 on experimental trichloroethylene (TCE) toxicity in rat ovaries by using immunohistochemical detection of malondialdehyde (MDA) in tissues as well as histopathological scores' computation. Material and Methods: Twenty-four healthy rats were randomly divided into four equivalent groups: control (sham-operated), TCE+vitamin D3 (1000 mg/kg/day and 50 IU/day, in that order), TCE (1000 mg/kg/day), as well as vitamin D3 (50 IU/day). Histopathological and immunohistochemical analyses were carried out on tissue samples 21 days following exposure to vitamin D3 and TCE. Results: Histopathological examination showed a significant decrease of angiogenesis in the corpus luteum and a substantial degeneration in the ovarian follicles and germinal epithelium in ovarian tissues of TCE group. Also, immunohistochemical examination for MDA immunoreactivity revealed that MDA immunoreactivity was significantly increased in the TCE group compared to the control group. We observed that vitamin D3 administration improved histological parameters, which include follicular degeneration as well as germinal epithelium degeneration, limiting TCE's negative impacts on ovarian tissues. It was also confirmed that MDA immunoreactivity was considerably reduced in ovarian tissues in the TCE + vitamin D3 group. Conclusion: In this study, the significant improvements observed in immunohistochemical and histopathological tissue oxidative stress parameters indicate that treatment with vitamin D3 could be a conservative method to prevent ovarian TCE toxicity.
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